DNA Melting Temperature Calculator

DNA Melting Temperature Calculator: Calculate DNA Tm

Calculate the melting temperature (Tm) of a DNA sequence. Input your DNA sequence (using A, T, C, G) using the keypad or keyboard, then click Calculate to get the Tm in degrees Celsius.

How It Works

Enter your DNA sequence using the keypad or keyboard, then click “Calculate” to see the melting temperature. The calculator displays the total sequence length and Tm (°C). Use the “Share” button to copy the calculator’s URL or the “Embed” button to copy an iframe code for embedding on another website.

DNA Melting Temperature Calculator

DNA Sequence

Result

Total Sequence Length:

Melting Temperature (°C):

Steps

About DNA Melting Temperature Calculator

The DNA Melting Temperature Calculator computes the melting temperature (Tm) of a DNA sequence, which is the temperature at which half of the DNA duplex dissociates into single strands. It’s a useful tool for molecular biology research, such as PCR primer design.

How to Use

  • Enter your DNA sequence using the keypad buttons (A, T, C, G) or keyboard (case-insensitive).
  • Click the “Calculate” button to compute the Tm. The page will scroll to the result.
  • Click the “Clear” button to reset the sequence input and results.
  • Click the “Share” button to copy the calculator’s URL to your clipboard for sharing.
  • Click the “Embed” button to copy an iframe code for embedding the calculator on another website.
  • The total sequence length and Tm (°C) will be displayed, along with detailed step-by-step calculations below.

Formulas

Melting Temperature (Tm):

Tm (°C) = 2 × (A + T) + 4 × (G + C)

Important Notes

  • The sequence must contain only valid nucleotides: A, T, C, G (case-insensitive). Invalid characters or empty sequences will trigger an error.
  • The Tm is calculated using the Wallace rule, suitable for short sequences (≤50 bases) under standard conditions (50 mM Na+).
  • The “Share” and “Embed” buttons require a browser that supports the Clipboard API.